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KMID : 0380619740060040231
Korean Journal of Food Science and Technology
1974 Volume.6 No. 4 p.231 ~ p.240
Production of Single - Cell Protein on Petroleum Hydrocarbon
±ÇÅ¿Ï/Kwon, Tai Wan
¹ÎÅÂÀÍ/º¯À¯·®/Mheen, Tae Ick/Pyun, Yoo Ryang
Abstract
The growth of a mixed yeast culture consisting of Canda tropicalis var. KIST 76 and Tricosporon cutaneum KIST 76-H was compared with that of pure cultures under pilot plant conditions. The mixed culture was judged stable based on the nearly constant ratio of the two organisms at the completion of fermentation. We obtained higher cell yields, protein content and productivity in the mixed culture on n-paraffin than the pure culture of C. trepicalis var. KIST 76. T. cutaneum KIST 76-H did not grow on n-paraffin medium. With the batch cultivation of mixed organisms on n-paraffin, the specific growth rates during the exponential growth phase were 0.24-0.33 hr^(-1); cell
yields were 96-106 and productivities were 2.9-3.6g/§¤. hr. The cells obtained contained 55-58% crude protein and 5.5-6.3% lipid. The critical value of dissolved oxygen concentration Ccrit. and saturation constant, km, are approximately 1.5 ppm and 0.228 ppm respectively. Also we established the optimal conditions for the mixed culture in batch fermentation.
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